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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in several tumors, which ends up in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was identified to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a important job in advertising and marketing cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are already explained. Two added splice variants were being documented but detailed nucleotide sequence information and facts isn't nevertheless available.
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Blocking peptides are peptides that bind specially to your focus on antibody and block antibody binding. These peptide normally includes the epitope acknowledged with the antibody.
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This guide outlines more attainable optimization of mobile and immunoassay parameters to guarantee the very best success are...
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This tutorial outlines even further attainable optimization of cellular and immunoassay parameters to make sure the very best effects are...
Antibodies certain to the blocking peptide no longer bind into the epitope to the goal protein. By evaluating the staining through the blocked antibody compared to the antibody alone, you can see which staining is particular.
Focus on Details Cyclin E belongs towards the remarkably conserved cyclin loved ones, whose members are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins purpose as regulators of CDK kinases. Various cyclins exhibit distinctive expression and degradation patterns which lead to your temporal coordination of each mitotic celebration. Cyclin E sorts a complex with and features as a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S changeover. This protein accumulates in the G1-S stage boundary which is degraded as cells development as a result of S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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